The field of bioanalytical chemistry overlaps a diverse range of disciplines, including biotechnology, biopharmaceuticals, and diagnostics - all of whuch make a comprehensive introduction to the subject even more essential for students and researchers. The Second Edition of Bioanalytical Chemistry follows the same path of the First Edition by providing a thorough introduction to bioanalytical chemistry for students and practioners. In doing so, it brings together many of the techniques commonly used by biochemists and molecular biologists. Along with many other topics, the text includes entire chapters on design and implementation of enzyme assays, mass spectrometry, and validation of new methods. The significantly expanded new edition includes three new chapters and focuses on recent developments in bioanalytical chemistry, while minimizing some of the less relevant techniques. The authors have limited mathematical derivations to those that are essential for a basic understanding of the principles underlying each method.